Leica EM PACT2 High Pressure Freezer

Leica Updated: 2009-02-07 RSS
Leica EM PACT2 High Pressure Freezer

The Leica EM PACT2 High Pressure Freezer is the first step in the preparation of samples for many subsequent techniques, allowing high quality freezing up to 200 µm into the specimen.

The instrument only needs to be connected to a standard power supply and comes with its own compressor located on the same trolley making it fully mobile.

The Rapid Transfer System EM RTS is an accessory to Leica EM PACT2. It allows an ease of sample preparation never before experienced in the field of High Pressure Freezing. Place your sample in the flat carrier held in the fork and simply push it into the instrument. The EM RTS does the rest. It takes the sample, locks it into the pod and shoots it into the Leica EM PACT2 where it is frozen - all in less than 2.5 seconds!

Key Features

* Leica EM PACT2
* High cooling rates by strong jet of LN2
* Compact and mobile
* Standard electrical supply
* Compressor on EM PACT2 trolley
* Data download on memory stick
* Low LN2 consumption
* Dewar with drain outlet
* Universal hydraulic system - can even use water
* Leica EM RTS
* Specimen carrier loading in less than 5 seconds
* Correlative LM/EM
* Rapid biopsy process
* Control touch sensitive screen
* Rapid loader for inserting specimen
* Automatic and reproducible specimen freezing
* Low noise

Detailed Description

High Pressure freezing in the Leica EM PACT2 is the first step in the preparation of samples for many subsequent techniques, allowing high quality freezing up to 200 µm into the specimen. The instrument needs to be connected to a standard power supply and comes with its own compressor, located on the same trolley, making it fully mobile. Any fluid can be used in the hydraulic system and new specimen pods are maintenance-free. A touch-sensitive color screen with prompt windows makes its use very easy. After freezing, pressure and temperature curves can be observed, then stored and downloaded via a memory stick to a PC. A days work requires only around 25 litres of LN2. Removal at the end of the day is simple - just open the drain valve on the Dewar and dispense unused LN2 into your storage Dewar.

A variety of specimen carriers are available for the many samples you may encounter and for ease of handling in follow-on procedures.
Leica EM RTS
The Rapid Transfer System, EM RTS, is an accessory for the Leica EM PACT2. It allows an ease of sample preparation never before experienced in the field of high pressure freezing, minimizing user actions. Place your sample in a carrier held in the loading fork and simply push it into the instrument. The EM RTS does the rest. It takes the sample, locks it into the pod and shoots it into the EM PACT2 where it is frozen - all in less than 2.5 seconds! The EM RTS is designed for time resolution studies in conjunction with confocal microcopy.

A cell can be viewed under the confocal microscope, then quickly frozen with the EM PACT2/EM RTS, allowing the same cell to be viewed in the electron microscope. The EM RTS can also be used to enhance the speed of taking a biopsy in conjunction with the new Microbiopsy Transfer System. With a turn-around time of 30 seconds for the EM Pact2, this means samples can be frozen at twice the rate of any other high pressure freezer.